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Molecular docking As no experimentally resolved structure was available, the amino acid sequence of mouse PCBP1 (GeneID: 23983) was modeled using ProteinX, an open-source AlphaFold-like implementation, and the resulting structure was refined with Rosetta Relax to improve stereochemical quality and stability

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A negative regulatory protein, Keap1, has been shown under non-stressed conditions to keep Nrf2 in a silent state [23]